Posts Tagged ‘Medicine’

Fever dreams – the true tale of Richard Spruce

// March 26th, 2012 // 1 Comment » // Drugs

Richard Spruce had seen some strange villages since arriving in South America in 1849, but this one took the cake. It was a ghost town. Every door was shut tight against the hot, humid jungle, while inside people slumbered away the sunlight.

Being the adventurous sort, he couldn’t comprehend such laziness, not, that is, until he mopped his clammy brow. His hand returned smeared with squashed mosquitoes and his own bright red blood.

He reflected, not for the first time, that life in the jungle wasn’t all it was cracked up to be, all things considered. Charles Darwin had joyfully described the Brazilian rainforest as “a great wild, untidy, luxuriant hothouse.” The tales of Alfred Wallace, a fellow young botanist, absolutely dripped with adventure.

Money truly did grow on trees here – there was a fortune to be made by transporting unusual plant species to England, where new novelties for Victorian gardens fetched a pretty penny.

Plus, the trailblazers in taxonomy whispered, it was delightfully warm, warm being a most thrilling word to Brits.

Had they mentioned the innumerable insects? If so, he still hadn’t expected the particular, primal discomforts of living in a cloud of whining and dining mosquitoes. Likewise, he hadn’t realised that breathing the air’s rich humidity would be as drinking tepid whisky through a straw. Nor how hot the nights were, wrapped tight as a flower bud in his stockings and blanket with a handkerchief over the face to ward away bloodsucking bats.

Nonetheless, Richard Spruce was not one to fret. He was of unfazeable stock, and though inch of his bare skin was soon in welts (not to mention his unmentionables), he followed the river and its plethora of plants to plunder. The cloud of mosquitoes followed too.

It’s not surprising what happened next.

The fever came on suddenly; a shivering, sweating, aching fever that rendered him helpless, striking him down mid-step on the border of Venezuela and Colombia.

His guides carried him shaking to a village. He knew the symptoms, hell he’d seen it before, but he denied his own diagnosis. Though he had the cure in his pocket, quinine from the bark from the Cinchona tree, he was loath to take its bitter rescue. He didn’t want it to be malaria.

Like a pot of swamp water on the boil, was his brain, his temperature climbing like lianas, curling like fern fronds, perching like epiphytic orchids. Images sprung forth from his fertile mind. The first two days he flashed on those damn mosquitoes, a haze of infected blood cells bursting. It didn’t make sense! The mosquitoes had left him three days ago. Certainly it wasn’t malaria, certainly, for it had been weeks since he was around the bad air of stale water, giving the Italian term mal’aria. Still he kept seeing mosquitoes.

As the fever broke into freezing chills, Richard’s guides began to mutter. When those chills turned once more to fever, they sensed his impending demise and sold his scientific equipment for rum. The patient was in no condition to care.

Spruce was stuck on mosquitoes, thriving in stagnant water and stale air, their droning drilled through his brain. He shrunk to the size of a grain of pollen and was sucked up like whisky through a mosquito’s straw. Inside the mosquito gut (it sure was hot and sticky) blood cells burst to release hideous parasites. These sex cells, for he identified them thus as surely as an anther and stigma, combined inside the mosquito. In the gut wall they formed cysts full of eggs. Or were they seeds? Or ferns?

Whatever they were, they grew for over a week, and exploded (much like his mind) yielding youngsters that frolicked freely.

Richard wasn’t frolicking. By the twinges in his aching joints, he knew the pangs of an elderly mosquito carrying young parasites, which had moved to his salivary gland to yield virulent juices. Next time he ate, dipping his mosquito’s double straw through the skin, spitting and sucking simultaneously, he would administer his chemical cocktail – anaesthetics to dull the pain, anti-blood clotting agents and, of course, the parasites.

Through the whisky straw Richard swirled, straight to the liver. His own liver, human and wracked with heat. From the liver, parasites paraded to the blood cells. Inside they ate oxygen-carrying haemoglobin and ran round after round of asexual reproduction, like spores or strawberry runners, each strawberry red and juicy, dripping. Each round took three days to replicate, feast and rupture the blood cells, like clockwork, and his body followed the same ticking cycle, burning fever following freezing chills following fever. Tick. Tick. Tick.

Richard shivered.

From outside he heard the nurse employed for his care, drunkenly yell “die, you English dog, that we may have a merry valorio with your dollars.” Well may she want a valorio, or watch night, but Richard was no a corpse.

After fifteen days of dreaming fever, he relented. Malaria it was. He took the bark of Cinchona trees, which kept him alive (just) by reducing his extreme body heat and causing the haemoglobin-chomping parasites to choke on their own waste. Such sweet relief from such bitter bark.

Thirty-eight days after his collapse, Richard was alive, but exhausted. His full recovery took many months more though, naturally, he kept collecting plants once he found new equipment.

Trudging onwards, Richard felt naught but respect and gratitude for the fine tree, Cinchona (though he preferred moss as a general rule).

Ten years later, having bushwhacked his way through saucer-sized tarantulas and marching fire ants, Richard found himself in the Andes. Gone were the South American rainforests, here roamed high altitude winds and freezing snows. After so long in the heat, the extreme chilliness didn’t suit him at all. But, onwards and upwards, as they say, and he was here to hunt Cinchona trees.

The trees were in high demand by the British and Dutch, both needing supplies for their malaria-wracked colonies. They had no steady supply, as the species had never been cultivated. There were sincere concerns that people would harvest it into extinction.

Richard spent a cold, windswept year collecting seeds and growing young plants. Almost 700 seedlings, well wrapped in moss, were tended all the way to England by a gardener assigned to their care. From the survivors, more than two hundred thousand precious plants were sent on to grow in Indian plantations.

Richard’s success with the species that saved his life did nothing less than change the world, making the heart of Africa habitable and saving millions of lives – but in the end he paid for it with his own health. Another disease cost him the use of his limbs, and he spent the rest of his days on a small pension in Britain.

Today, malaria kills around two million people each year and infects 200 million more. It has quite possibly killed more people throughout history than all our wars and plagues combined. Quinine, along with other chemicals, is still used for cures and prevention, and is gathered from the decedents of Richard Spruce’s trees.

Drinking notes: Enjoy this true tale with warm whisky or gin and tonic. Small quantities of Quinine are added to some brands of tonic water for flavour. Fluorescent, the chemical glows under black light. Many thanks to highly informative Flower Hunters by Mary and John Gribbin for the biography of Richard Spruce.

Christmas chemistry, the science of holly

// December 21st, 2011 // 4 Comments » // Poisons

pudding with holly

Chocolate orange icecream pudding with side of holly. Image by webmink

Green and red, classic Christmas colours, adorn the spiky holly shrub. A sprig may garnish puddings, but garnish nibblers like me must hold back on holly for it is poisonous in large doses – though some leaves can make a tasty beverage!

Holly includes about 400 species in the genus Ilex. The cultivated species is Ilex aquifolium, and about 20 or 30 of those bright berries can kill an adult. Poisonings are more likely in pets or children, and about five berries will make a kid feel sick.

It’s the usual suspects in symptoms – sleepiness, sore tummy, vomiting, diarrhoea. Larger doses cause paralysis, kidney damage and death.

Chemically, they contain a cocktail of active ingredients. Among them are the triterpenes, precursors to steroids which are cytotoxic (kill cells), steroids and a nitrile called menisdaurin.

Traditional medicines use holly for fever, gout and chronic bronchitis.

Holly, image by 4nitsirk, flickr

A couple of species native to North America, I. vomitoria aka yaupon and I. cassine, make caffeine and were used to make “black drink”, a stimulating brew also used as a vomit-causing emetic.

South American species I. paraguariensis contains as much as 1.6% caffeine (five times more than the above species) and some of the cocoa chemical theobromine in their leaves, and tasty tannins.

Also called yerba mate, I. paraguariensis is brewed to make mate tea, which is delicious. It’s pronounced MAH-tay, but be careful not to put the emphasis on the second syllable. Wikipedia says that makaes mah-TAY, which means “I killed” in Spanish.

So it’s fine to have a sprig of holly in the house for Christmas, just don’t make a holly pie out of it!

Have a nap and let your computer cure cancer

// October 18th, 2010 // 2 Comments » // Drugs, How Things Work, Recent Research, Science Communication

computer doing science

Image by John Watson

While waiting for inspiration to strike a solid introduction into my head, my computer screen went blank. Good ol’ MacBook conserving energy! But letting your computer go idle doesn’t mean you have to waste its processing power. Why not cure cancer with grid computing?

It’s a kind of parallel computing, which breaks up complex problems into smaller calculations and then solves them at the same time. Instead of one processor working on one calculation a time, a group of processors work on different calculations together. Dual-core computers is one way to do it. Grid computing is another.

Grid computing is like a massive virtual computer whose processors are computers linked by a central software.

World Community Grid is one group which utilises the personal computers of over half a million volunteers around the globe. Their software switches on when the computer is idle and runs virtual experiments, calculating and number crunching its way through chemical simulations. They provide this public grid to humanitarian research projects.

Childhood cancer
One of the projects they are running is helping to solve childhood cancer by finding potential new drugs for neuroblastoma, one of the most common solid tumors in children. In some people the tumors do not respond well to chemotherapy. This research is hoping to turn this around by targeting three proteins which are important to the cancer’s survival. Knock out those proteins and the cancer will in turn be knocked out by chemo.

Good plan, but how to knock out the proteins? That’s where the grid comes in.

There are three million potential drug candidates who MIGHT bind to one of the proteins and knock them out. Of course, that’s a lot of laboratory time right there. A computer would be better, but to run these nine million virtual experiments would take 8000 years. By working with the public grid they expect the project to be finished in just two years. Possibly less.

That’s a big saving on time and grant money. It’s rational based drug design (which I blogged about here) taken to a crowd sourcing extreme. They are trying a similar thing to discover dengue fever drugs.

Carbon Nanotubes

Image by Mstroeck

Clean Water
Drug design isn’t the only industry using the World Community Grid. Last month universities in Australia and China announced they are running simulations through the grid to find out how to filter water using nanotubes.

Nanotubes are small tubes that only water molecules can fit through. Not bacteria, not even viruses. It’s a great way to get rid of water dwelling nasties and desalinate sea water. But with such small pores you would expect the pressure and energy needed to force water through the filter to be incredible. And incredibly expensive. But in 2005 experiments showed that actually the water flowed pretty fast through the filters.

Why? Possibly the water molecules touching the nanotubes act more like ice and reduce friction. But who knows? To find out exactly what’s happening they’re running realistic simulations using the grid. The outcome could lead to huge improvements in water availability, potentially saving millions of lives a year in the developing world.

Like the idea of grid computing? Sign up to the World Community Grid here, and let your down time make a difference.

Noble Prize in Chemistry – Palladium catalysed reactions

// October 6th, 2010 // 1 Comment » // How Things Work, Science Communication

Image adapted from Jurii

The winners of this years Nobel Prize in Chemistry goes to Richard F. Heck, Ei-ichi Negishi and Akira Suzuki, for their work in palladium catalysed reactions.

Ah, a subject close to my own heart! As a student of Molecular and Drug Design, we studied this shizz in lectures. Hell, I think I even did a Suzuki reaction! That pretty well makes me famous IMHO.

SO – palladium catalysed reactions. What are they, I hear you say? Oh, dear gentle reader, how long do you have for me to BLOW YOUR MIND WITH CHEMISTRY AWESOME? Three minutes? K.

Carbon to carbon bonds are super important in the human body, which is pretty much made of carbon. Nitrogen, hydrogen and oxygen get a look in, but carbon is where it’s at.

There’s a big trend at the moment, has been for years, in designing small molecules as drugs. Some small molecules mimic the molecules naturally inside the body. Basically it’s telling the body what you to do in a language it can understand.

To make a carbon-based small molecule, you need to make some carbon to carbon bonds. The sad part is that carbon is a chiller, and isn’t keen on making friends with other carbons. Put a carbon and another carbon in a test-tube and they just won’t get it on. They don’t care to so much as hold hands.

HOWEVER, chuck some palladium catalyst into the mix and ba-zing! You’ve got yourself a sweet, sweet reaction that’s controllable and would otherwise have taken a zillion years to happen. Now we can create new molecules and drugs to benefit peeps everywhere!

Words cannot describe how nerdy and happy I am right now to write about palladium catalysed reactions. Maybe I’ve missed my calling as a chemist after all.

Day One Nobel Prize Week – Father of IVF wins Nobel for Medicine

// October 5th, 2010 // No Comments » // Science Communication

This week is vegetarian week, and it’s also the week Nobel Prize winners are announced. Coincidence? I think not.

The 2010 prize for Physiology and Medicine was awarded to Robert G. Edwards for the development of in vitro fertilisation.

His initial success in creating a blastocyst outside the womb happened in Cambridge in 1968. The world’s first IVF baby was born ten years later in 1978. Since then the number of children conceived by IVF are around four million.

I find IVF incredibly interesting. It has not only given children to millions of parents, it’s also responsible for SCIENCE STUFF. Like countless discoveries into how embryo’s grow, and how to improve health during pregnancy.

I’ll keep you updated on the winners for Physics and Chemistry.

Medical dictionary translates English to Yolngu Matha

// September 13th, 2010 // 2 Comments » // Science Communication

Yolŋu Matha is a language spoken by the Indigenous Australians of Arnhem Land, in the Northern Territory. To the majority of the people in the communities, English is a second language. There’s a twelve year gap in life expectancy between Indigenous people and non-Indigenous people, which is pretty drastic. It’s made worse because there’s a massive communication gap between the doctors and patients.

Just last week, ARDS released a new dictionary that translates medical phrases into Yolŋu Matha. Here’s some examples:

DNA – djinaga’puy wäyuk or djinawa’wuy wäyuk
English: DNA is found inside every cell of our body. It acts like a law that is not easily changed. It controls what kind of cell each cell grows into e.g. a skin cell, or liver cell or brain cell. It also controls what work each cell does.
Yolŋu Matha: Dhuwaliyi ŋunhi djinawa’wuy wäyuk, ŋunhiny ŋayi ŋuli ga ŋorra ŋunhan bili yan ṉapuŋgan ŋunhiliyin ŋunhi nhänhamiriw waka’ rumbalwu yäku cell-ŋura dhuwal rumbalŋura limurruŋgal. Ga rommirr ŋayi dhuwaliyi djinawa’wuynydja wäyuk, ŋunhi ŋanya dhu bäyŋun nhakun yuwalktja rrorru’. Ga buŋgawayirrnydja ŋayi ŋuli ga ŋunhi bukmakkun dhiyak cell-wuny mala nhaltjan ŋayi dhu walalany dhanuŋdhun rommirriyam balanya nhakun: ŋanakpuy dhuwal rumbalpuy cell-nha ga bamburuŋburuŋbuynha cell-nha ga biḏila’puynha cell-nha. Ga ŋunhi ŋayi ŋuli goŋ-dhawar’yundja bala ŋayi ŋuli djämamirriyaman ŋunhi cell-nhany mala

hormone – dhäwu-gänhamirr wiyika’

English: Hormones are substances that are produced in our body and carried by our blood. Each hormone has its own message to give to our body.
Yolŋu Matha: “Hormone”-dja dhuwal wiyika’ mala ŋunhi ŋuli ga ŋamaŋamayunmirr dhiyal rumbalŋur limurruŋgal, ga gämany walalany ŋuli ga ŋunhi maŋguy’nha. Ga bukmakthu “hormone”-dhu ga gäna-gana ŋayatham dhäwu mala ŋunhi walal ŋuli ga gurrupan dhipal bukmaklil rumballil limurruŋgal.

How awesome is that?

Antibiotic beer, as drunk by the ancient Nubians

// September 8th, 2010 // 4 Comments » // Drugs, Recent Research

Image by Peter Trimming

Today’s schooner of science is literally science in a schooner. Plus it comes with a new career path – bioarcheologist, expert in ancient diets.

George Armelagos is the bioarcheologist in question, and he’d been studying the ancient Nubians who lived just south of ancient Egypt in present-day Sudan.

George was looking at some bones and found evidence that they had been exposed to tetracycline, an antibiotic. Tetracycline is absorbed into bone, and fluoresces green. It’s sometimes used to measure bone growth – take tetracycline at day 0, again at day 12, and at day 21 take a biopsy. The distance between the two green lines will show how far the bone grew in 12 days.

Anyhoo, tetracycline in bones from 350-550 AD is weird, seeing as we first invented antibiotics with the discovery of penicillin in 1928. Now we find out the ancient Nubians beat us to it, and as with all great ideas they came up with it over a beer.

The grain they used to ferment the beer contained streptomyces bacteria, which produces tetracycline as a kind of germ warfare. Like penicillin comes from a fungus, tetracycline is made by a bacteria. It’s a bad-ass antibacterial that can treat disease like chlamydia, gonorrhea, and pneumonia which are caused by bacteria. It can even kill Yersinia pestis cause of the black plague.

Were the ancient Nubians drinking it by accidental contamination, intentional medication, or did streptomyces bacteria just grew on the corpses?

To find out they needed (da dada dum!) a CHEMIST! This particular hero was Mark Nelson, who dissolved the bones in some hardcore hydrogen fluoride – “the most dangerous acid on the planet,” according to Mark. Woah. After showing the bones who was boss, Mark mass spec’d the shizz out of them and discovered a metric buttload of tetracycline, confirming that it was ingested and in high quantities.

The scientist duo concluded that this was a brew with a purpose – an antibiotic alcoholic. Even the bones from a four year old child contained a lot of tetracycline, perhaps he was given the antibacterial to cure a disease.

My question is, why are WE not taking our antibiotics in beer? That would be SO much better!

ResearchBlogging.orgNelson ML, Dinardo A, Hochberg J, & Armelagos GJ (2010). Brief communication: Mass spectroscopic characterization of tetracycline in the skeletal remains of an ancient population from Sudanese Nubia 350-550 CE. American journal of physical anthropology, 143 (1), 151-4 PMID: 20564518

Apothecary bottles found in a collectibles shop

// August 21st, 2010 // 2 Comments » // Drugs

About a week ago I was in Gulgong, a small town in New South Wales near the wine region of Mudgee. The main road was spelled Mayne Road, and was brown stone rather than tarmac. Along the footpaths were old stone troughs for watering horses. Key landmarks included the Ten Dollar Motel and the Gulgong Butchers Cafe. It was an old gold mining town which had lost its gold but kept its rural charm.

Wandering the streets I came across a collectibles shop filled with coloured glass jugs and gold rimmed plates. Amongst the copper kettles I found these old bottles from an apothecary, dated around the 1800′s I believe.

Old Apothecary Bottles

The craftsmanship is stunning, and they teased my imagination. What were these drugs used for? What did they look like, when those bottles were filled, and who was the chemist who filled them?

I have since looked into some of the medicines written on the bottles.

Iodoformum is now called tri-iodomethane (CHI3). The crystals are lemon yellow and have a disagreeable odour and taste. I think it was used to treat tuberculosis, and is still used in homeopathy for a range of ailments. Hexamine may have been mixed with hippuric acid to make methenamine hippurate, which was used to treat lower urinary tract infections. Salol was a white powder derived from salicylic acid, the active ingredient in willow bark, which we take as acetylsalicylic acid in asprin. It was used to reduce pain and fever. Menthol you probably recognise from chest rubs. It comes from mint oil, though it can be made synthetically. As well as clearing sinuses it can ease sore throats and muscle pains, and is one of the ingredients in tiger balm.

While researching I found an issue of the British Medical Journal from September 5, 1885 which is an interesting read.

World’s sweetest antibiotic? The five ways honey kills bacteria.

// July 13th, 2010 // 5 Comments » // Drugs, How Things Work, Recent Research, Science at Home

HoneyYou’re at the doctors with a suspected infection, but instead of offering penicillin or erythromycin, they prescribe honey. Would you switch toast toppings? Take a honey pill? How about letting the doctor smear medical grade honey over the infected area?

People have been using honey (not mad honey) as medicine since ancient times, but until now we have never fully understood how it works. Research lead by Dr. Paulus Kwakman from the University of Amsterdam and his team have finally identified the key elements which give honey its antibacterial activity.

Bacteria are becoming resistant to drugs faster than we’re developing them. Honey might help because it works when other drugs don’t. Studies show it has good activity in vitro against antibiotic-resistant bacteria. An older study reports successful treatment of a chronic wound infections not responding to normal medicine.

So how does it work? It’s a combination of five factors.

1. Hydrogen peroxide, a kind of bleach. The honey enzyme called glucose oxidase makes hydrogen peroxide when honey is diluted with water. We clean toilets with bleach, and it’s pretty good at killing bacteria.

2. Sugar. Honey has so much sugar there’s hardly any water for bacteria to grow in.

3. Methylglyoxal (MGO), an antibacterial compound found in New Zealand Manuka honey a couple of years ago. It’s also found in medical grade honey which is made in controlled greenhouses, albeit in smaller amounts.

4. Bee defensin 1, a protein found in royal jelly (special food for queen bee larva.) This report is the first time bee defensin 1 has been identified in honey, and it works as an antibiotic.

5. Acid. Diluted honey has a pH of around 3.5, the acidic environment slows down bacterial growth.

These five things work together to provide a broad spectrum activity against bacteria. For example, S. aureus is vulnerable hydrogen peroxide, while B. subtillis is challenged only if MGO and bee defensin 1 are working simultaneously. Honey has the right mix for maximum destruction.

So that’s how bees keep their honey fresh and unspoiled by bacterial growth. Perhaps with this information we’ll create enhanced honey to guard against infection, improving on nature like we did with penicillin. Until then, I know what I’m having on my toast.

A Schooner of Science could be named Australia’s best science blog. If you enjoyed reading, please vote for me.

ResearchBlogging.orgKwakman, P., te Velde, A., de Boer, L., Speijer, D., Vandenbroucke-Grauls, C., & Zaat, S. (2010). How honey kills bacteria The FASEB Journal, 24 (7), 2576-2582 DOI: 10.1096/fj.09-150789

How to stop coughing with medicine or chocolate

// May 26th, 2010 // 4 Comments » // Drugs

Image by Peter Pearson

I have been sadly struck down with a cold. So it was with weary steps I took myself to the pharmacy to obtain suitable drugs, and plenty of them. I got capsules which contain paracetamol (painkiller,) phenylephrine (ineffective speed-like drug that used to be pseudoephedrine and now doesn’t really work) and dextromethorphan (cough suppressant.)

Well I’m still coughing… So let’s see what the deal is with Mr. Dextromethorphan. Dexter for short.

Dexter is chemically similar to morphine and codeine. It was first developed to replace codeine as America’s favourite cough suppressant, and has been successful because it is non-addictive and has less potential for abuse. Unfortunately there’s debate about whether it actually works, particularly in children.

It may have less potential for abuse, but people still have a good time on cough medicine. Too much Dexter acts as a dissociative hallucinogen similar to ketamine (aka Special K) or PCP. Possibly you could enjoy watching a TV show of animals through a wide angled lens if you had too much.

If that’s not your cup of tea, why not actually have a cup of tea? Tea contains theobromine, which can suppress coughing. You know what else has theobromine in it? Cocoa. Dark chocolate in particular is a good source of theobromine, and is a delicious alternative to cough medicine. The study reported by New Scientist showed it was more effective than codeine, and better than placebo.

Dexter must have kicked in now, I’m not coughing so much. I always feel spaced out from cough medicine though, and I’m feeling pretty zonked… Methinks I will seek out some large supply of chocolate, just in case.